Whole Genome Sequencing

Overview:

Whole Genome Sequencing provides a complete and unbiased view of the genome, covering both coding and non-coding regions. Sequencing is performed using short-read (Illumina) or long-read (Oxford Nanopore) platforms, depending on the required resolution and read length. Coverage depth can be adjusted depending on the application, typically from 30x to over 100x. Applicable to genomic DNA extracted from blood, cells, tissues or other samples. For Nanopore long-read sequencing, high molecular weight DNA is essential. It enables comprehensive detection of single nucleotide variants (SNVs), indels, copy number variations (CNVs), structural rearrangements and large chromosomal alterations. WGS supports population and evolutionary genomics, disease gene discovery, cancer genome profiling, de novo genome assembly, microbial strain typing and full characterization of reference strains or cell lines, among other applications.

Genomic DNA extraction, DNA fragmentation, library preparation, optional indexing/multiplexing, sequencing (short- or long-read).