Amplicon / Deep-Targeted Sequencing

Overview:
Amplicon sequencing selectively amplifies predefined genomic regions through multiplex PCR, producing highly specific libraries with minimal input material (as low as 1 ng). Amplicon workflows are compatible with both Illumina short-read platforms and Oxford Nanopore long-read platforms (e.g., for full-length amplicons or viral genomes). Depth routinely exceeds thousands of reads per amplicon, enabling detection of rare variants, making this approach ideal for microbial strain typing, viral sequencing, mitochondrial genome sequencing, HLA typing, CRISPR editing validation, and low-input or degraded samples. While cost-efficient and highly sensitive, amplicon sequencing is limited by PCR biases, incomplete coverage of GC-rich regions, and an inability to detect structural variants outside the targeted loci. Nonetheless, it offers fast, accurate, and scalable targeted interrogation of selected genomic sites.
Workflow:
PCR amplification of target regions, library preparation, sequencing.

Service options:

• Library prep only
• Sequencing only
• Library prep + sequencing
• Genomic Extraction + Library prep + sequencing