Targeted bisulfite sequencing

Overview:

Targeted Bisulfite Sequencing focuses on predefined genomic regions, such as promoters, CpG islands, regulatory elements, or gene panels, allowing high-depth methylation analysis with reduced sequencing cost. After bisulfite conversion, targeted regions are enriched using amplicon-based or hybrid-capture methods and sequenced on Illumina platforms to achieve very high depth—often >500x to >2,000x depending on panel size and experimental needs. This approach requires high-quality genomic DNA (10 ng to several hundred ng depending on the kit) and is well suited for clinical research samples, FFPE tissues, low-input specimens, or targeted epigenetic profiling. It enables precise detection of methylation changes in specific regulatory loci, supports longitudinal monitoring of epigenetic alterations, and is widely used for validation of genome-wide epigenomic screens, targeted biomarker development, epigenetic diagnostics research, tumour methylation profiling, and cell-type or tissue-of-origin studies, among other applications.

Workflow:

Genomic DNA extraction, bisulfite conversion, targeted enrichment (amplicon PCR or capture), sequencing.